Effect of aging on human induced pluripotent stem cell generation – UROP Spring Symposium 2021

Effect of aging on human induced pluripotent stem cell generation

Jerry Juratli


Research Mentor(s): Nadia Sutton, Assistant Professor
Research Mentor School/College/Department: Internal Medicine- Cardiology, Michigan Medicine
Presentation Date: Thursday, April 22, 2021
Session: Session 2 (11am – 11:50am)
Breakout Room: Room 12
Presenter: 5

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Coronary stents are used to relieve patient symptoms of angina, or chest pain, due to obstruction of blood flow in coronary arteries. After stents are implanted, endothelial cells grow over the stents. Before that occurs, patients are at risk for developing a clot, or thrombus, inside of the stent. We are seeking to find novel ways to accelerate the coronary stent endothelialization process. We aim to understand if human-induced pluripotent stem cells (hiPSC-ECs) are a viable option for pre-endothelializing stents prior to implantation. In addition to aiming to determine stent growth properties on coronary stents, we aim to discover if there is a phenotypic difference between the endothelial cells created from hiPSC-ECs taken from younger and older patients by using a sample of their mononuclear blood cells. We are currently taking blood samples from older subjects having a procedure in the cardiac catheterization lab. After obtaining consent for the patient, approximately 8 mL of the patient’s blood is drawn from the patient’s IV into a vial with an anticoagulant. The vial is centrifuged for 30 minutes at 1800 RCF in order to isolate the mononuclear blood cells. Afterward, the sample is transported to the Cardiovascular Regeneration Core Laboratory, where the cells are converted to hiPSC-ECs and placed on commercially-available coronary artery stents to observe their growth. Thus far, one blood sample has been recently collected, with results from the cardiovascular regeneration core lab pending. Separately, subjects in the young age group will be recruited through the www.UMHealthResearch.org website. In total, we aim to test cells from blood samples acquired from 20 different patients over the course of the year. Potentially, hiPSC-ECs from older patients can be reprogrammed to more closely resemble cells from younger patients.

Authors: Jerry Juratli, Andre Monteiro Da Rocha, Todd Herron, Allison Schley, Nadia Sutton
Research Method: Laboratory Research

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