Sejal Patil
Pronouns: she/her
Research Mentor(s): Qiao Li
Co-Presenter:
Research Mentor School/College/Department: Surgery / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 3 – 1:40pm – 2:30 pm
Room: League Ballroom
Authors: Sejal Patil, Ying Xin, Jiayu Liu, Qiao Li
Presenter: 92
Abstract
Recently it was reported that tumor cells secrete exosomes that express PD-L1. Additionally, suppression of exosomal PD-L1 could induce systemic anti-tumor immunity leading to decrease of tumors in mice. We are testing if the suppression of exosomal PD-L1 can induce anti-melanoma immunity using a murine melanoma cell line D5 in C57BL/6 mice. The cell lines used for this purpose include Rab27a knockout (Rab27ako) D5 cells, PD-L1 knockout (PD-L1ko) D5 cells, as well as wild-type (WT) D5 cells. These cells were cultured in vitro to examine their proliferation. The D5 Rab27ako cells have exosome deleted; the D5 PD-L1ko cells have PD-L1 deleted, and both cell lines are expected to have a different response in immunocompetent normal mice with the existence of an immune system due to the absence of exosomes and PD-L1. However, when we cultured these cells in vitro without the involvement of immune cells and factors, we found that they grow in the culture flasks similarly to the growth of D5 WT cells, indicating that the knockout of either Rab27a or PD-L1 did not change the biological activity of the D5 cells. This results will allow us to use these genetically modified, e.g. Rab27a knockout and PD-L1 knockout D5 cells to further investigate the effect of the exosomal PD-L1 on tumor growth in vivo when these cells are injected into the C57BL/6 mice. D5 WT cells will be used as a control. These experiments will also help understand the immune responses and mechanisms against exosomal PD-L1 in cancer immunotherapy.
Biomedical Sciences, Interdisciplinary, Natural/Life Sciences
