Lucy Potts
Pronouns: she/her
Research Mentor(s): Dipankar Ray
Co-Presenter:
Research Mentor School/College/Department: Radiation Oncology / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 1 – 10am – 10:50am
Room: League Ballroom
Authors: Lucy Potts, Dipankar Ray
Presenter: 46
Abstract
Esophageal adenocarcinoma(EAC) is a cancer growing in relevance. Barrett’s esophagus cells which result from mutation in normal esophagus cells often from esophagus damage are very likely to develop into EAC. Past studies have evaluated the changes that occur as BE cells progress to EAC. One change that occurs is as cells progress from BE to EAC isoform expression of RNF128 is altered as mRNA expression for Iso1 is increased and mRNA for Iso2 is decreased. Iso2 of RNF128 is a cancer suppressor, aiding in reducing levels of mutant p53, while Iso1 is oncogenic and stabilizes mutant p53. Our goal is to revert the RNF128 isoform expression back to their BE levels or prevent the isoform expression switch. A potential mechanism for this isoform switch is miRNA gene expression regulation. The two isoforms of RNF128 are identical in sequence, except in the first exon. miR590 was a microRNA identified with a binding site on exon 1 RNF128 Iso2. Lab experiments transfected CpA and HepG2 cells with inhibitors for both the 3p and 5p forms of miR590. Protein and RNA analysis was then performed to evaluate the resulting isoform expression from this treatment.
Biomedical Sciences, Interdisciplinary, Natural/Life Sciences
