Apichaya Sethaudom
Pronouns: she/her
Research Mentor(s): Ruma Banerjee
Co-Presenter:
Research Mentor School/College/Department: Biological Chemistry / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 3 – 1:40pm – 2:30 pm
Room: League Ballroom
Authors: Apichaya Sethaudom, Ruma Banerjee, Roshan Kumar
Presenter: 95
Abstract
Endothelial cells line the heart and blood vessels and are important in angiogenesis, which is the process by which new blood vessels are formed. Angiogenesis is essential for delivery of nutrients and oxygen and therefore for growth and wound healing. H2S, which is produced by endothelial cells induce angiogenesis. However, when the H2S concentration is too high, it inhibits cell growth. The aim of this study is to understand the role of H2S in endothelial cells, using the human EA hy926 cell line as a model. Genes involved in regulating H2S levels in cells (i.e. gamma-cystathionase (CSE), cystathionine beta-synthase (CBS), sulfide quinone oxidoreductase (SQOR) and persulfide dioxygenase (ETHE 1) were knocked down and their effects on cell proliferation were examined. Cell number was determined using a Cellometer at four time points (24, 48, 72 and 96 h). Endothelial cells with CSE, CBS and SQOR knockdown (KD) exhibit reduced proliferation. Although H2S levels were expected to decrease in the CSE and CBS KD cells, H2S was actually observed to increase. When pyruvate was added to SQOR KD cells, proliferation was rescued, which is consistent with pyruvate increasing the concentration of electron acceptors. When LbNOX is expressed in the mitochondria of SQOR KD cells, they proliferate more than the control cells or those expressing LbNOX in the cytoplasm. This observation is consistent with the effects of H2S being targeted to the mitochondrion, and, specifically, the electron transport chain.
Biomedical Sciences, Interdisciplinary
