Kayla Krusinga
Pronouns: she/her
Research Mentor(s): Anna Mathew
Co-Presenter:
Research Mentor School/College/Department: Internal Medicine/ Nephrology / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 5 – 3:40pm – 4:30 pm
Room: League Ballroom
Authors: Kayla Krusinga, Biaoxin Chai, Anna Mathew
Presenter: 3
Abstract
Chronic Kidney Disease (CKD) is gradual and irreversible damage of the kidney and is prevalent in 15% of Americans. Past studies have shown patients with CKD are at 10-40 times higher risk for cardiovascular disease than the general population. Changing cholesterol levels and behaviors such as smoking and exercise to prevent heart disease in CKD patients because macrophage-derived inflammation and oxidative stress play a more significant role. Indoleamine 2,3-dioxygenase, an enzyme catabolizing tryptophan, is upregulated in the vascular-wall macrophages of CKD patients and is involved in the propagation of inflammation and oxidative stress. We hypothesize that indoleamine 2,3-dioxygenase (IDO-1) plays a role in apoptosis and cytokine production and therefore influences the progression of cardiovascular disease in kidney patients. We utilized primary macrophage cell cultures from murine models that underwent genetic manipulation of IDO-1. We measured the levels of apoptosis and cytokines using ELISA and Immunoblot assays. We find that the inhibition of IDO-1 in primary activated murine macrophages increased 47 of the 111 cytokines tested by the murine cytokine proteome profiling assay. We also find that deletion of IDO-1 increased apoptosis levels in these cultures. Our findings add evidence to the role of IDO-1 in macrophage-derived inflammation in the context of cardiovascular disease associated with CKD.
Biomedical Sciences, Interdisciplinary, Natural/Life Sciences
