Zubaida Azeeza
Pronouns: she/her
Research Mentor(s): Isabelle Lombaert
Co-Presenter:
Research Mentor School/College/Department: Biologic & Materials Sciences / Dentistry
Presentation Date: April 20
Presentation Type: Oral5
Session: Session 5 – 3:40pm – 4:30 pm
Room: Breakout Room 5
Authors: Zubaida Azeeza, Christina Jones, Isabelle Lombaert
Presenter: 1
Abstract
Salivary gland dysfunction is a major adverse effect of therapeutic irradiation (IR), a typical treatment used for head and neck cancer patients. Unfortunately, there are no long term solutions to repair IR-damaged salivary glands. Our lab studies Sox10, a key transcription factor in controlling the expansion and differentiation of epithelial stem/ progenitor cells, as a potential target for a stem cell-based solution to repair salivary glands. Previously, our lab has shown that Histone Deacetylase 1 (Hdac1), an enzyme involved in chromatin remodeling and transcription regulation, and Sox10 interact with one another in epithelial cells in the salivary glands. It is uncertain how Hdac1 and Sox10 interact and how that affects epithelial stem cell differentiation, so we hypothesize that Hdac1 and Sox10 binding is required to induce stem cell differentiation. To determine how Hdac1 and Sox10 bind each other, we will use a mouse ductal cell line (SIMS) transfected with truncated Sox10-Myc-DKK tagged plasmids and verify Hdac1-Sox10 binding using a Myc immunoprecipitation (IP) and Hdac1 Western Blot (WB). These techniques will allow us to determine which plasmids maintain Hdac1-Sox10 binding, specifically which functional domain of Sox10 is required for Hdac1 to bind. Overall, this study aims to identify the function of Hdac1-Sox10 binding in order to inform future novel cell therapeutics to stimulate tissue regeneration for patients that suffer from xerostomia and other adverse effects of IR-damaged salivary glands.
Biomedical Sciences, Interdisciplinary, Natural/Life Sciences
