Prashanti Donthireddy
Pronouns: she/her
Research Mentor(s): Brian Byrd
Co-Presenter: Rollins, Annabelle
Research Mentor School/College/Department: Medicine / Cardiology / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 3 – 1:40pm – 2:30 pm
Room: League Ballroom
Authors: Satish Rao, Prashanti Donthireddy, Annabelle Rollins, James Brian Byrd
Presenter: 43
Abstract
A large proportion of the American population consumes large amounts of salt regularly. However, the impact of these high levels of salt is unknown. In the Sodium Diet Effect on Aldosterone and Urinary RNA (SALTY) study, we asked healthy, young, normotensive adults to consume a low-salt diet (230 mg/day) and a high-salt diet (6900 mg/day) (in random order) to understand if differences exist in the concentrations of various metabolites in samples taken during the high-salt period compared to the low-salt period. Initial urine and blood samples were taken prior to starting the diets. Subsequently, participants were randomized to eating the high or low-salt diet first. After completing the first component of the diet, a 6-day wash out period occurred before the participants consumed the next component of the diet. The urine and plasma samples were processed and analyzed using high performance liquid chromatography – tandem mass spectrometry (HPLC-MS/MS) to identify various metabolites within the blood plasma and urine samples. The mass spectrometry data was run through Metaboanalyst 5.0 software to generate the final results and determine the differences in metabolites between low and high salt dietary conditions. There were significant differences in the concentrations of various metabolites between the high and low salt diets. Taurine in plasma and glutathione in urine were shown to be significantly dysregulated between high-salt and low-salt diet treatments. We are in the process of identifying other molecules that are dysregulated. These metabolomic data allow for the identification of specific metabolic pathways that may be up- or down-regulated in the presence of excess salt. Understanding these metabolic pathways can provide useful insight into the specific processes that occur in the body as a result of consuming high levels of salt.
