Nanopore Electroporation in Microfluidic Systems for Dynamic Delivery of Macromolecules into Cells – UROP Spring Symposium 2023

Nanopore Electroporation in Microfluidic Systems for Dynamic Delivery of Macromolecules into Cells

Andrew Schaefer

Andrew Schaefer photo

Pronouns:

Research Mentor(s): Sasha Cai Lesher-Perez
Research Mentor School/College/Department: Chemical Engineering / Engineering
Program: UROPF
Session: Session 6 (3:40pm – 4:30pm)
Authors:

Abstract

This paper presents the development of a control for microdevices designed to incorporate laminar flow within nano-electroporation. The purpose of this control is to improve the transfection rate and homogeneity of microfluidic devices and revolutionize drug testing by reducing the time needed for experimental drug trials. The microfluidic devices consist of a PDMS base, micrometer channels, and a transfusing membrane. The authors focused on designing and testing a machine to print PDMS onto membranes, and they developed two types of controls, one with structures and the other with sectioned-off membranes, to showcase the uses of nano-electroporation with laminar flow. Overall, this paper presents the successful creation of a control for microdevices, which can improve the performance of microfluidic devices and enhance the efficiency of drug testing and controlled biorhythms.

Engineering

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