Nathaniel Ayoub
Research Mentor(s): Nadia Korovesis
Mentor Department: Department of Biomedical Engineering
Authors: Domingo Uceda, Victoria Sturgess, Nadia Korovesis, Ali Citalan-Madrid, Katherine Stangis, Nathaniel Ayoub, Eric Vu, Sal Esajee, Vibujithan Vigneshwaran, Gregory Sands, Dan Lawrence, Daniel Beard, Johnathan Tune, Geoffrey Murphy, C. Alberto Figueroa
Session: Session 5 (2:00pm – 2:50pm)
Presentation Type: Poster 75
Abstract
This study presents the optimization of a CUBIC-based tissue clearing protocol for three-dimensional imaging of myocardial microvasculature in swine without requiring vascular perfusion. Through systematic refinements in reagent incubation timing, we studied the optimal incubation times (short vs prolonged) that maximize tissue transparency while preserving endothelial marker integrity. Quantitative assessment will demonstrate which reagent incubation times improved imaging depth and signal-to-background ratio compared to standard protocols. This refined method enables detailed characterization of intact microvascular networks at subcellular resolution, providing valuable insights into the three-dimensional architecture of cardiac microvasculature and allowing for segmentation and physiological modeling.
