Nicholas Kirana
Research Mentor(s): Jianzhi Zhang
Mentor Department: Ecology and Evolutionary Biology
Authors: Nicholas Kirana, Yunju Lee
Session: Session 3 (11:00am – 11:50am)
Presentation Type: Poster 73
Abstract
CRISPR-Cas9 genome editing has revolutionized functional genomics and synthetic biology by enabling precise and efficient genetic modifications across a wide range of organisms. However, success rate has not been fully optimized to reach high percentages and this is especially prevalent in the case of high-throughput library construction—where large numbers of genetic modifications are introduced and screened in parallel. Several researchers have determined the current most successful rate of editing to be stagnant at 74% after having tested a couple of variables. In this research experiment, variables such as length of the homologous arms, the length of donor DNA, concentration of DNA, concentration of Cas9 plasmid, loci, yeast strain, ploidy, cell growth stage, initial cell number, and cutting site of Cas9 will all be tested to improve efficiency. It is hypothesized that if a certain combination of these variables are used to optimal efficiency then the success rate of the CRISPR-Cas9 system will significantly increase. This is especially important as it will help researchers in the field who are currently working with yeast and the CRISPR-Cas9 system and can pave a way for more efficiency.
