Dysfunction of insulin producing pancreatic beta cells – The Central Problem in Diabetes – UROP Spring Symposium 2021

Dysfunction of insulin producing pancreatic beta cells – The Central Problem in Diabetes

Alexander Deck

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Pronouns: He, Him, HIs

Research Mentor(s): Mehboob Hussain, Professor
Research Mentor School/College/Department: Internal Medicine, Metabolism, Endocrinology, Diabetes, Michigan Medicine
Presentation Date: Thursday, April 22, 2021
Session: Session 5 (3pm-3:50pm)
Breakout Room: Room 7
Presenter: 5

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Abstract

This lab and experience over the past school year has involved two separate components. The first component was in a wet lab doing actual work. The work I was doing was genotyping different mice and their offspring to test for genetic mutations. This was done through a tail snip being taken from the mice and digested to release the DNA. The DNA is then placed in a polymerase chain reaction (PCR) to replicate the DNA strand. The results of this PCR were then ran through a Gel electrophoresis system in order to measure the base pair lengths of genes being investigated. This in person lab research coincides with the auxiliary research I was doing. I was told to investigate how Beta cells measure the glucose level of blood and release the correct corresponding amount of insulin. This knowledge can then be used to see if certain genetic mutations in different mice result in differing insulin production or even a stop of insulin production.

Authors: Alexander Deck
Research Method: Laboratory Research with Animals

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