Research Mentor(s): Somin Eunice Lee, Assistant Professor
Research Mentor School/College/Department: Electrical Engineering and Computer Science, College of Engineering
Presentation Date: Thursday, April 22, 2021
Session: Session 3 (1pm-1:50pm)
Breakout Room: Room 2
The use of non-viral vectors to deliver the CRISPR/Cas9 complex to target cells is beneficial as they can potentially negate the immunological response that a host may have to the viral vector used in the present. In order to validate this, one must first identify the optimal guide RNA for the Cas9 complex to the target DNA sequence, which is done through PCR amplification and gel electrophoresis. The next phase is to image the cells by immunofluorescence. The transfected cells were then compared to the baseline cells to visualize the effects that the CRISPR/Cas9 complex on the cells.
Authors: Brett Silber, Pramith Senaratne, Ann Gladstone, Xintao Zhao, Somin Lee
Research Method: Laboratory Research