Teresa Lee
Pronouns: She/Her
Research Mentor(s): Alvaro Rojas Pena
Co-Presenter:
Research Mentor School/College/Department: Department of Surgery-Transplantation / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 2 – 11am – 11:50am
Room: League Ballroom
Authors: Teresa Lee, Yeniselis Morales, Gergely Lautner, Mark Meyerhoff, Steven Schwendeman, Robert H. Bartlett, Alvaro Rojas-Pena, Orsolya Lautner-Csorba
Presenter: 12
Abstract
Patients that require intense cardiovascular care may require the extracorporeal membrane oxygenator (ECMO). This mechanism allows blood to be “cleaned,” oxygenated, warmed, and then circled back into the patient; this process may result in thrombus formation. One of the risks of using regulated anticoagulants like systemic heparin to prevent thrombus is undesired bleeding. Another method to prevent thrombus formation would be to modify the blood-encountering polymer surfaces within the ECMO circuit that releases nitric oxide (NO), which has antithrombic properties. Our study focuses on the development and optimization of a new solvent impregnation method by impregnating only the inner surface of the tube with a novel, lipophilic RSNO type NO donor, SNAT. First we characterized any changes of surface morphology of the treated extracorporeal circuit (ECC) with Scanning Electron Microscopy (SEM). We also measured the NO release profile of the treated ECC with ozone chemiluminescence. The effect of different storage temperatures and sterilization were also tested. The SEM surface characteristics did not differ significantly between the NO releasing and the untreated tube. The normal physiological range for NO release is 0.5-4×10^-10 mol/min/cm^2 (NO flux unit), which mimics the environment of the endothelium lining of the blood vessel. The in vitro test showed that the circuit had >2 flux unit over a 42 day period. The OPA Plus liquid chemical sterilization decreased the NO release by 20% compared to the non-sterilized ECC. After one month long storage, the samples stored at -20? and 4?, exhibited the highest NO flux on day 1 of measurement compared to the ECC stored at room temperature (12.3 vs. 13.9 vs. 6.6 NO flux unit, respectively). The NO release either exceeded or fell within the target physiological range (0.5-4 flux) over a prolonged period of time. This study with the novel, optimized solvent impregnation technique can expand on the anticoagulation palette of surface modifications in extracorporeal circuit application.
Biomedical Sciences, Interdisciplinary
