Madison Buckles
Pronouns: She/Her
Research Mentor(s): Kurt Hankenson
Co-Presenter:
Research Mentor School/College/Department: Orthopaedic Surgery / Medicine
Presentation Date: April 20
Presentation Type: Poster
Session: Session 2 – 11am – 11:50am
Room: League Ballroom
Authors: Madison Buckles, Kurt Hankenson, Heather Wilson
Presenter: 70
Abstract
The Notch signaling pathway plays an important regulatory role during the process of bone regeneration. This project focuses on the molecular mechanisms of the Notch pathway and its interactions with BMP. BMP is another growth factor that promotes bone formation, which is currently used clinically. However, BMP has serious negative side effects. The goal of gaining a better understanding of BMP signaling in relation to Notch signaling is to help improve patients’ treatment options in the future. This project aims to demonstrate that Notch signaling is necessary locally during BMP induced bone regeneration and to characterize the impact of Notch signaling on mesenchymal progenitor cells expansion and differentiation during BMP mediated bone defect healing in mice. The Hankenson lab has shown in vitro that BMP signaling and Notch signaling interact with each other. The lab uses a mouse calvarial defect model to study Notch signaling and bone formation in vivo. Mouse calvarial defect models are used to study bone healing because they allow the study of intramembranous bone formation and Noth signaling at the defect sites. Our hypothesis is that, building upon studies in vitro, Notch signaling is required for activation and expansion in mesenchymal progenitor cells during BMP-induced calvarial bone regeneration. Future studies will investigate the Notch and BMP pathways in osteoblastogenesis and further study the intersection of these two pathways in animal models.
Interdisciplinary, Natural/Life Sciences
