Hedi Liu
Pronouns:
Research Mentor(s): Dawen Cai
Research Mentor School/College/Department: Cell and Developmental Biology; Biophysics; Neuroscience Graduate Program / Medicine
Program:
Authors:
Session: Session 2: 10:00 am – 10:50 am
Poster: 42
Abstract
Neural network imaging is a critical aspect of neural studies, particularly for delving into the intricate connectivity within intact mouse brains. However, the light-scattering properties inherent in brain tissues present challenges to imaging depth, necessitating the use of optical clearing techniques for a thorough analysis. To address this challenge, we employ innovative chemical and biological strategies to minimize refractive index mismatches between lipid-rich mouse brain tissue and the imaging sample solution. Our experimental gel framework, designed to support the entire mouse brain, encompasses several essential steps, including mouse perfusion, tissue permeabilization, protein cross-linking, whole-brain gelation, and enzyme-assisted delipidation, all aimed at optimizing the clearing of brain tissue. The clarity of the entire mouse brain depends significantly on precise control over the selection of gel monomer and crosslinker, as well as the detergent participation during the clearing process. By extending immunofluorescence imaging depths to millimeters and achieving comprehensive coverage of the entire mouse brain, in conjunction with automated microscopy and advanced imaging processing tools, we anticipate achieving unparalleled resolution and scalability in tracking and reconstructing neuronal networks.
