Samantha Loan
Pronouns: she/her
Research Mentor(s): Mohamad Tiba
Research Mentor School/College/Department: Emergency Medicine / Medicine
Program:
Authors: Shawna Lee
Session: Session 7: 4:40 pm – 5:30 pm
Poster: 97
Abstract
The comprehension of sepsis progression and its physiological implications remains elusive, largely due to inadequate models that effectively mirror human bodily reactions. To mitigate model disparities, the introduction of large animal models possesses the capability to encapsulate data pertaining to the multifaceted systemic impacts of sepsis. The goal of this specific project is to study the pathophysiological basis of sepsis using a previously established model (Tiba et. al., 2020). The study aims to measure when and how various organ systems decline during sepsis. For the sepsis model, swine models were anesthetized, mechanically ventilated, and surgically instrumented for continuous hemodynamic and serial blood sampling (Tiba, et al). Surgical instrumentation is initiated with a laparotomy to access various organ systems. Lines are placed in a combination of veins and arteries for continuous vital monitoring and blood samples. The animals were introduced to a uropathic pathogen strain of E. coli through direct infusion into the renal parenchyma, beginning a 24 hour monitoring period. Post monitoring period, tissue samples are taken during necropsy for sectioning and the animal is euthanized. As this project is still in progress, results have not yet been obtained and conclusions have not been reached. The sepsis project will add to the big picture of emergency medicine research because it will create a better understanding of the pathophysiological mechanism of sepsis. The model aids in deepening our comprehension of the mechanisms underlying infection-induced multi-organ dysfunction and failure. Its application holds promise for bridging the gap between laboratory discoveries and clinical practice in sepsis research, refining resuscitation methods, and assessing potential treatments.
