Matthew Nho
Pronouns: he/him
Research Mentor(s): Ahmed Abdel-latif
Research Mentor School/College/Department: Internal Medicine-Cardiology / Medicine
Program:
Authors: Ahmed Abdel-Latif, Afnan Alzamrooni Alzamrooni, Rajesh Chaudhary
Session: Session 1: 9:00 am – 9:50 am
Poster: 98
Abstract
Myocardial infarction (MI) results in permanent tissue loss and scar formation due to a cardiac immune response triggered by ischemic injury. Repairing the damaged heart after MI is the ultimate objective in cardiovascular research. We utilize Acomys mice, known for their unique systemic tissue regenerative capacity, to investigate the mechanisms behind their enhanced cardiac repair. We hypothesize that Acomys macrophages promote myocardial preservation after MI by releasing protective factors that contribute to their regenerative potential. Bone marrow cells were isolated from the bones of 8-week-old Acomys and Mus species. After sacrifice, the bones were surgically removed and mechanically cleared of muscle and tendon. Marrow was aspirated from bones by flushing with sterile PBS through a 26-gauge syringe. Red blood cells were lysed and the remaining cells were plated and grown for six days in DMEM supplemented with L929 media. After six days in culture, bone marrow derived macrophages (BMDM) were plated and allowed to settle for 24h before activation with IFN-? and LPS for 24h. Supernatant was collected and assayed for inflammatory markers using the ELISA technique. Transcriptional profiling of Acomys macrophages identified factors that facilitate regeneration (unpublished data). Our findings reveal distinct inflammatory phenotypes in Acomys macrophages not observed in Mus. In conclusion, our study provides insight into the role of macrophages in regenerative species, shedding light on strategies to enhance cardiac repair and regeneration in Acomys following MI. Understanding the mechanisms governing macrophage-mediated tissue remodeling is crucial to bridging the knowledge gap between regenerative medicine and clinical medicine.
