Ruchir Sriram
Pronouns: he/him
Research Mentor(s): Jie Ren Gerald Har
Research Mentor School/College/Department: Biologic and Material Sciences and Prosthodontics / Dentistry
Program:
Authors: Ruchir Sriram, Maggie Hsu, Emily Zhu, Gerald Har, Lauren Surface
Session: Session 4: 1:40 pm – 2:30 pm
Poster: 34
Abstract
It is known in the medical and scientific community that Chronic Kidney Disease (CKD) negatively impacts bone health in humans. A person who has CKD is more likely to have fractures, and these fractures also take longer to heal. However, little is known about the biological mechanisms that cause poorer bone function due to CKD. This study aims to explore how CKD impacts bone function and metabolism by using mouse models. Biochemical assays of the urine and serum of CKD-induced mice compared to those of healthy mice were performed and analyzed to assess kidney function. Metabolic tracing experiments have been performed on the bone of CKD-induced mice. Preliminary results show that the TCA cycle, and potentially mitochondrial function, are altered in multiple organs, including the bone, in CKD-induced mice against healthy mice controls. Further experiments using histological staining will confirm the results obtained through the assays and tracing. Cell culture will also be performed in-vitro with osteoblasts exposed to high phosphate to study how the osteoblast cell will change on a molecular level in response to the phosphate. The results from this study will have significant implications for developing bone therapies to help people with CKD and weak bones.
