Neenor Razoky
Research Mentor: Orsolya Lautner-Csorba
Mentor Department: Urop Ecls Student, Medicine
Author(s): Not Available
Session: Session 5 (2:00 PM – 2:50 PM)
Presentation Type: Poster 92
Abstract
Abstract Title: Engineering NO-Releasing Hemodialysis Catheters for Infection Prevention Author List: Neenor Razoky, Ryan Kauffman, Anthony Cinetti, Jianfeng Wu, Andrew Hill, Josef Hill, Alvaro Rojas-Pena, Orsolya Lautner-Csorba Background: Hemodialysis is a life-sustaining therapy for patients with end-stage renal failure but is frequently complicated by catheter-related bloodstream infections (CRBSIs), thrombosis, poor blood flow, and inflammation. Current antimicrobial catheter technologies, including silver-impregnated and chlorhexidine-coated devices, and antibiotic treatment have limited effectiveness against biofilms. Nitric oxide (NO), an endogenous signaling molecule, exhibits antimicrobial, antithrombotic, and vasodilatory properties, making it a promising candidate for improving catheter performance. This study aims to develop a nitric oxide–releasing hemodialysis catheter using the highly hydrophobic S-nitroso-1-adamantanethiol (SNAT) NO donor capable of sustained NO release in order to prevent CRBSIs.. Methods: Catheter segments were prepared by solvent-impregnation with 1000 mg/mL SNAT in hexane. Their mechanical integrity (Young’s modulus) was evaluated using a texture analyzer (TA) to ensure preservation of catheter strength after treatment. NO release profiles were quantified using a highly sensitive ozone chemiluminescent analyzer. Antimicrobial efficacy was assessed against Staphylococcus aureus and Pseudomonas aeruginosa bacteria strains through bacterial killing (2h, 4h, 6h, 8h, 24h) and biofilm assays (1 day, 3 days, and 14 days). Student t tests were performed to determine the significance of the results (p < 0.05). Results: Hexane-treated SNAT catheters maintained the original mechanical properties (2.4 +/- 0.68) comparable to untreated controls (2.6 +/- 0.001) while achieving adequate NO release levels above the physiological targets of 0.5 x 10-10 mol min -1 cm-2 for up to 14 days. Treated catheters demonstrated significant reductions in planktonic cell counts and biofilm formation, providing 2.5-3 log reductions. Conclusion: These results demonstrate that the SNAT-impregnated hemodialysis catheter offers sustained NO release for up to 14 days and displays significant antimicrobial effects. These findings suggest that the SNAT-impregnated hemodialysis catheter offers a promising strategy to prevent CRBSIs and improve long-term hemodialysis catheter performance.
