Yunying Zhang
Research Mentor: Theodore Goodson III
Mentor Department: Chemistry, LSA
Author(s): Bishal Saha, Yunying zhang, Yunying Zhang, Theodore Goodson III
Session: Session 7 (4:00 PM – 4:50 PM)
Presentation Type: Poster 24
Abstract
The binding mechanisms of DNA-targeting drugs play an important role in the development of pharmacological research. These drugs typically interact with DNA through intercalation or groove binding and produce different, distinct effects upon binding. Although spectroscopy techniques like CD (circular dichroism) have been used in the past to analyze these interactions, the results are often ambiguous. Classical two photon absorption was found to be an effective alternative to find the exact binding mode of the drugs through spectroscopy, but it was found that it was not efficient through microscopy as it leads to ambiguity. We propose the use of quantum microscopy for better clinical analysis of the drugs in vivo. In our quantum microscope we use entangled two photons as an excitation source to produce fluorescence images so (ETPFM) offers the opportunity for more detailed analysis. Our research uses entangled two-photon fluorescence microscopy to examine the interaction between drug and DNA, specifically analyzing the binding behavior and differentiating drugs by their binding type: intercalate or groove binding. By using ETPFM, we aim to differentiate intercalate and groove-binding mechanisms in various compounds. Preliminary data indicate that this technique effectively distinguishes binding modes, demonstrating strong potential as an analytical method for drug development research.
