Xinyu Li
Pronouns: She/Her
Research Mentor(s): Nadejda Bozadjieva Kramer
Research Mentor School/College/Department: Surgery / Medicine
Program:
Authors: Xinyu Li, Nadejda Bozadijeva-Kramer, Jae Hoon Shin
Session: Session 6: 3:40 pm – 4:30 pm
Poster: 43
Abstract
Regenerating islet-derived protein 3g (Reg3g), a gut peptide known for its multifaceted roles in host defense and various physiological functions, has recently gained attention in the context of metabolic disorders. While its importance in responding to such disorders is emerging, the specific function of pancreatic Reg3g in glycemic control remains elusive. In this study, we will explore the protective role of Reg3g in the pancreas to maintain glucose homeostasis. By investigating the potential of Reg3g as a regulator of hyperglycemia, we strive to contribute to the development of less invasive therapies for weight loss.We generated the cohort of WT and Reg3g KO mice. Mice will be subdivided into 4 groups; chow diet and vehicle, inulin diet and vehicle, chow diet and STZ (streptozotocin), and inulin diet and STZ. Then we will monitor body weight and blood glucose levels weekly. We will also perform a glucose tolerance test (GTT) for 3 weeks and collect tissues 4 weeks post STZ injection for further analysis. Methodologically, we will use RNA isolation, cDNA synthesis, and quantitative PCR (qPCR) techniques to analyze gene expression and ß-cell morphology. We anticipate that this study will highlight the specific impact of pancreatic Reg3g on hyperglycemia regulation.This research carries significant implications for individuals struggling with impaired glycemic control, offering potential alternative therapies to current treatment options. By shedding light on the mechanisms underlying hyperglycemia regulation and its association with weight gain, this study aims to provide valuable insights into improving health outcomes for affected individuals.
